Health
Covid-19 or Dengue? Health news and top stories on how the NTU team can find out in 36 minutes
Researchers at the Recontian Medical School at Nanyang Technological University (NTU) have figured out how to find out whether a person is Covid-19 or Dengue in just 36 minutes.
The NTU said yesterday that this was only a quarter of the time it took with the current methods of testing for the same disease.
At this time, the most sensitive method for detecting Covid-19 is to use lab polymerase chain reaction (PCR) technology. Traces of the Sars-CoV-2 virus that causes Covid-19 can be detected.
However, while accurate, these methods can take several hours to complete due to a step called RNA purification.
When a patient is tested with a swab, his RNA needs to be extracted from the swab sample to remove substances in the sample that interfere with the function of the PCR test, such as mucin, the major component of mucus.
It also needs the chemicals that are currently in short supply around the world and must be performed by highly trained staff using expensive equipment.
However, the NTU team’s method, known as “Direct PCR,” uses a series of commercially available enzymes and reagents that are resistant to inhibitors to overcome this obstacle.
By mixing them in a test tube with the patient’s swab sample, the team can skip the RNA purification step and run the test directly on the sample to get accurate results in less time. I will.
Dr. Shivalingham Paramarine Gum Suppier, a Senior Research Fellow who is part of the team, said: Testing is on the rise and demand is growing globally. “
The team method can also be placed outside the laboratory using a machine called a portable thermocycler.
This means that the test method may be used by frontline healthcare professionals in the local healthcare setting without having to send the results to the lab for processing there.
From the lab to the front line
Our goal is to develop ultra-fast automated tests that deliver results in minutes and can be performed by clinicians in the same precision and sensitivity as professional laboratories. This allows you to perform PCR tests from traditional laboratories closer to the point of care and put them into the lowest resource settings you need most.
Associate Professor ERIC YAP, Team Leader.
Cost reduction
You can save money by skipping the RNA extraction step with the direct PCR method. It also avoids reagent shortage issues as lab testing increases and demand grows worldwide.
Dr. Shivalingham Paramaringum Sappia, Senior Researcher.
Team Lead Associate Professor Eric Yap pointed out that further research is needed to see how effective the test environment is in a real environment.
“When one bottleneck is removed, other challenges may emerge, such as ensuring quality control and reducing manual errors,” he said.
They are currently aiming to use this method in the NTU Clinical Diagnostic Laboratory Covid-19 test.
Professor Yap said the team’s method was also effective against the dengue virus, with results in just 28 minutes. “Since Singapore is countering the double outbreak of dengue and Covid-19, both of which have similar initial symptoms, our tests could help distinguish the two infections,” he said. Said.
“Our goal is to develop ultra-fast automated tests that deliver results in minutes and can be performed by clinicians in the same precision and sensitivity as professional laboratories.
“This allows us to move PCR tests from traditional laboratories to the healthcare setting and put them in the environment where they need the most resources.”
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