Connect with us

Health

New tablet-sized devices can profile multiple COVID-19 antibodies and biomarkers at the same time

Published

on

 


The test method can distinguish severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Type with 100% accuracy to predict case severity and resistance to variants.

A biomedical engineer at Duke University in the United States has developed a device that detects coronavirus disease (COVID-19) antibodies and a biomarker that distinguishes between antibodies produced in response to SARS-CoV-2 and four other antibodies. Developed. Coronavirus With perfect accuracy.

Image Credit: Corona Borealis Studio / Shutterstock

Newly adapted platforms can be crucial in the fight against SARS-CoV-2 and its variants

A flexible, cost-effective and accurate “D4 assay” platform has been adapted by Duke University scientists to help distinguish infection patterns from various variants of SARS-CoV-2.

The “D4 Assay” describes a platform for point-of-care clinical diagnostics based on four Ds. Specifically, the D4 assay contains four consecutive events: (2) Dissolution (reagent printed on the chip); (3) Diffusion (entire surface); (4) Detection (binding event).

D4 technology uses a polymer brush coating that acts as a kind of non-stick coating to prevent anything other than the desired biomarker from sticking to the test slides when wet, making the screening process extremely sensitive. It provides a very effective particle filter. In fact, D4’s major antibody (Ab) is composed of microarrays that can be quantified with picomolar sensitivity within 30 minutes.

The current version of the platform does not require electricity to process. With just one drop of blood and biomolecular lubricant, automated tests run in minutes, with results that can be read by a detector about the size of a very thick iPad.

Recently, the platform has been shown to detect Ebola infection one day earlier than the standard polymerase chain reaction (PCR) test. And now, researchers say the results show how flexible the technology is to adapt to other current and future illnesses, including SARS-CoV-2.

It took six years to develop the D4 assay, but when WHO declared the outbreak of a pandemic, we started to compress all that work into months and how the test would be a public health tool. I was able to find out if it could be used for. ” “Our tests are designed to achieve both adaptability and a true point of care,” said Ashtosh Chilkoti, a prominent professor of Alan L. Pehrsonov and chair of Duke’s biomedical engineering. The diagnosis will be most useful. “

A team of researchers has come to work to adapt the D4 assay to COVID-19. They showed that this test was able to distinguish between antibodies produced in response to SARS-CoV-2 and the other four coronaviruses with 100% accuracy.

Data was procured in three parts of SARS-CoV-2 for testing Effectiveness of Assay; subunit of Spike protein, Binding domains within peplomer and nucleocapsid proteins that package viral RNA.

The results found antibodies in all 31 tested patients, reporting 41 false positives from healthy individuals and antibodies from 18 samples from 4 other coronavirus-infected individuals. I showed that I did.

An important tool for identifying, tracking and addressing the emergence of new variants

“There are many questions from people about whether they are protected from new variants of COVID-19. Our tests can answer some of them,” says working at the Chilkoti lab, which developed the chip. Jake Heggestad, a PhD student, said. test.

Our platform should be able to distinguish whether we have antibodies that can neutralize the new mutants of concern, or if those antibodies are not protective against the new mutants, “the team said. Stated.

Based on preliminary findings, researchers are now wondering if this easy-to-use, energy-independent point of care device can be used to predict a person’s immunity to the severity of COVID-19 infection or variants. I’m trying to judge. Virus.

It is clear that adapting the assay can prove to be particularly valuable in tracking the appearance of potential mutants, especially in areas of high infection rate.

As we are platform builders, we are working to show how we can easily modify this technology to do a lot of things, “said David Kinnamon, a graduate student who developed a liquid processing system for testing. Stated. A single platform can act as a diagnostic, assess the immune response after infection, and predict the outcome of the disease. There aren’t many tests that can do that. “

Promising results from this study provide new candidates for integration into a broader pilot strategy for current and future disease outbreaks, especially those with high mutation potential or rapid infection. This is especially true because the D4 assay provides a cost-effective and reliable method that can be made widely available.

In fact, Heggestad describes the platform as “very user-friendly and portable.” “Doing all this in a centralized facility like Duke is one thing, except that you can run large-scale tests and get excellent and sensitive results in remote locations around the world. That’s it. “

Therefore, the best way to integrate such tests on a larger scale remains a challenge.

Journal reference:

  • Multiplexed Quantitative Serological Profiling of COVID-19 from Blood by Point of Care Test “, Jacob T. Heggestad, David S. Kinnamon, Lyra B. Olson, Jason Liu, Garrett Kelly, Simone A. Wall, Solomon Oshabaheebwa, Zachary Quinn, Cassio M. Fontes, Daniel Y. Joh, Angus M. Hucknall, Carl Pieper, Jack G. Anderson, Ibtehaj A. Naqvi, Lingye Chen, Loretta G. Que, Thomas Oguin III, Smita K. Nair , Bruce A. Sullenger, Christopher W. Woods, Thomas W. Burke, Gregory D. Sempowski, Bryan D. Kraft, Ashutosh Chilkoti Science Advances, June 25, 2021. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7654894/..

..

What Are The Main Benefits Of Comparing Car Insurance Quotes Online

LOS ANGELES, CA / ACCESSWIRE / June 24, 2020, / Compare-autoinsurance.Org has launched a new blog post that presents the main benefits of comparing multiple car insurance quotes. For more info and free online quotes, please visit https://compare-autoinsurance.Org/the-advantages-of-comparing-prices-with-car-insurance-quotes-online/ The modern society has numerous technological advantages. One important advantage is the speed at which information is sent and received. With the help of the internet, the shopping habits of many persons have drastically changed. The car insurance industry hasn't remained untouched by these changes. On the internet, drivers can compare insurance prices and find out which sellers have the best offers. View photos The advantages of comparing online car insurance quotes are the following: Online quotes can be obtained from anywhere and at any time. Unlike physical insurance agencies, websites don't have a specific schedule and they are available at any time. Drivers that have busy working schedules, can compare quotes from anywhere and at any time, even at midnight. Multiple choices. Almost all insurance providers, no matter if they are well-known brands or just local insurers, have an online presence. Online quotes will allow policyholders the chance to discover multiple insurance companies and check their prices. Drivers are no longer required to get quotes from just a few known insurance companies. Also, local and regional insurers can provide lower insurance rates for the same services. Accurate insurance estimates. Online quotes can only be accurate if the customers provide accurate and real info about their car models and driving history. Lying about past driving incidents can make the price estimates to be lower, but when dealing with an insurance company lying to them is useless. Usually, insurance companies will do research about a potential customer before granting him coverage. Online quotes can be sorted easily. Although drivers are recommended to not choose a policy just based on its price, drivers can easily sort quotes by insurance price. Using brokerage websites will allow drivers to get quotes from multiple insurers, thus making the comparison faster and easier. For additional info, money-saving tips, and free car insurance quotes, visit https://compare-autoinsurance.Org/ Compare-autoinsurance.Org is an online provider of life, home, health, and auto insurance quotes. This website is unique because it does not simply stick to one kind of insurance provider, but brings the clients the best deals from many different online insurance carriers. In this way, clients have access to offers from multiple carriers all in one place: this website. On this site, customers have access to quotes for insurance plans from various agencies, such as local or nationwide agencies, brand names insurance companies, etc. "Online quotes can easily help drivers obtain better car insurance deals. All they have to do is to complete an online form with accurate and real info, then compare prices", said Russell Rabichev, Marketing Director of Internet Marketing Company. CONTACT: Company Name: Internet Marketing CompanyPerson for contact Name: Gurgu CPhone Number: (818) 359-3898Email: [email protected]: https://compare-autoinsurance.Org/ SOURCE: Compare-autoinsurance.Org View source version on accesswire.Com:https://www.Accesswire.Com/595055/What-Are-The-Main-Benefits-Of-Comparing-Car-Insurance-Quotes-Online View photos



Pictures Credit

ExBUlletin

to request, modification Contact us at Here or [email protected]