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Stanford’s new 5-hour DNA sequencing technology sets Guinness World Records

Stanford’s new 5-hour DNA sequencing technology sets Guinness World Records

 


Many of the current innovations in the healthcare field are aimed at reducing the time to diagnosis, but by the time patients receive a definitive answer to their mysterious health, until test results and genetic analysis are complete. It may take days or weeks.

But thanks to a group of scientists at Stanford University School of Medicine, an elusive same-day diagnosis may finally be within reach.

Researchers at the university, led by Euan Ashley, a professor of medicine, genetics, and biomedical data science, have developed a rapid genomic sequencing approach that can accurately analyze an individual’s complete DNA composition in just five hours.

As a result, the time to diagnosis is greatly reduced.A dissertation on team work Published this week The New England Journal of Medicine describes how to sequence a patient’s entire genome and provide a diagnosis in just 7 hours and 18 minutes. This is almost twice as fast as the previous 14-hour recording set by Radiy Children’s. Sequencing-based diagnostic laboratory.

The sequencing part of the analysis was completed in just 5 hours and 2 minutes. This is a feat certified by the Guinness World Records to win the first title of the fastest DNA sequencing technology.

Related: Roche announces Avenio system for automating DNA sequencing sample preparation

To develop a “mega-sequencing” approach, a team at Stanford University used Oxford Nanopore Technologies and a new DNA analyzer that included 48 sequencing units. Scientists used all 48 flow cells at once to perform long read sequences, injecting subsequent onslaught of genomic data into cloud-based storage systems.

Once in the cloud, the algorithm gives the data a first path, highlighting mutations in an individual’s genes that indicate a potential disease. The research team then performed their own analysis to compare the identified variants with those known to cause the disease.

On average, with this ultra-fast technology, the entire sequencing and diagnostic process took only eight hours, but it’s still not fast enough for Ashley and his team.

“I think we can halve it again,” Ashley said. statement.. “If you can do that, you’ll get an answer before the ward round is over. It’s a dramatic jump.”

Related: Oxford Nanopore Invests £ 150m, Oracle Wins Genome Sequence Cloud Support

As explained in the newly published study, the team used a new approach to sequence the genomes of 12 patients. They were able to detect the genetic disease in 5 patients. This is about 12% higher than the standard for diagnosing mysterious illnesses.

These cases included a 13-year-old case of heart failure whose sequencing process revealed mutations in genes related to cardiac function. He was put on the transplant list shortly after the discovery, received a new heart within three weeks, and a year later, now his mother told Stanford.

Scientists are now planning to bring the technology to nearby intensive care units at Stanford Hospital and Lucille Packard Children’s Hospital Stanford before expanding to other hospitals.

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