Health
Influencing factors in SARS-CoV-2 nucleic acid detection
Violin diagram of CT value distribution for Qualitative Proficiency Test (PT). CT values of ORF1ab and N of samples D and E (A). CT values of ORF1ab in sample D using different nucleic acid detection kits (B), different participants (C), and different instruments (D). Fewer than three categories were not included in the statistics. credit: biosafety and health (2022). DOI: 10.1016/j.bsheal.2022.08.004
The COVID-19 epidemic continues to ravage the world. It is worth discussing the different factors that influence SARS-CoV-2 nucleic acid detection (NAT) and the importance of quantitative and qualitative detection capabilities, and some factors are new to improve testing capacity. Suggested in the article. biosafety and health Provide guidance for containing the spread of the outbreak.
Some agencies are conducting qualitative SARS-CoV-2 proficiency tests for domestic laboratories, raising issues such as lack of overall testing capacity and lack of personnel training. However, quantitative capabilities and regulatory regimes are rarely discussed.
In terms of quantitative performance, dPCR methods are more comparable and reliable, whereas qPCR results are easily influenced by standard material usage and personnel manipulation. Quantitative competence is the basis for qualitative judgment. Participants from hospitals reported more satisfactory results, whereas participants from industry were less satisfied.
Systems such as “CE” certification based on “self-declaration” are temporarily unsuitable for China, and national supervision and development of various verification projects will play an important role in improving nucleic acid detection capabilities. .
Not all laboratories perform satisfactorily with SARS-CoV-2 tests, especially ICL tests. Quality systems need to be further improved, including selection of qualified test kits, personnel training, standardized operations, routine quality control, and regular external evaluations.overall improvement test ability It greatly avoids the occurrence of laboratory test errors and helps prevent and control epidemics.
Yongzhuo Zhang et al., Evaluation of Factors Contributing to Variability in Qualitative and Quantitative Competence Tests for SARS-CoV-2 Nucleic Acid Detection, biosafety and health (2022). DOI: 10.1016/j.bsheal.2022.08.004
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Quote: Impact Factors in SARS-CoV-2 Nucleic Acid Detection (October 10, 2022) will be published on October 10, 2022 at https://medicalxpress.com/news/2022-10-factors-sars-cov-nucleic- Taken from acid.html
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