Connect with us


Research provides insights into bacterial natural product molecular factories


The active agents of many drugs are so-called natural products, because only microorganisms are often able to produce complex structures. Similar to factory production lines, large enzyme complexes bring these activator molecules together. The teams at the Technical University of Munich (TUM) and the Goethe University of Frankfurt have succeeded in investigating the basic mechanism of one of these molecular factories.

Many important drugs, such as antibiotics and active agents against cancer, are natural products made by microorganisms such as bacteria and fungi. In the lab, these natural products are often not produced at all or only with great effort. Many starting points for such compounds are polyketides. Polyketides are carbon chains that are double-bonded to the oxygen atom every second atom.

It is produced in microbial cells such as Photorhabdus luminescens bacteria with the help of polyketide synthase (PKS). In the first step of the PKS type II system, four proteins come together to change a “team” to build a molecule of interest step by step.

In the second step, they are further enzymatically modified to the desired natural product. Examples of natural products of bacteria produced in that way are, inter alia, clinically used tetracycline antibiotics or doxorubicin, anti-cancer agents.

Interdisciplinary cooperation

The modified step of the second stage has been well studied for many active agents, but to date, the first stage of these molecular factories in which highly reactive polyketide intermediates are attached to the enzyme complex has been investigated. There was little insight into its general functioning, and it is protected against natural reactions.

This gap is closed as a result of a collaboration between the working group of Michael Groll, a professor of biochemistry at the Technical University of Munich, and Helg Bode, a professor of molecular biotechnology at the University of Goethe in Frankfurt. Natural chemistry..

Discovery Inspires New Synthesis of Activators

In connection with this work, for the first time, we have analyzed the complex of various partner proteins of the type II polyketide synthase with the aid of X-ray structural analysis, in order to gain a detailed understanding of the complete catalytic cycle. became. “

Michael Groll, Professor of Biochemistry, Technical University of Munich

“Based on these findings, it will be possible in the future to target and manipulate central biochemical processes, thus altering the basic structure instead of being restricted to decorative enzymes,” Helge Bode. He adds.

While developing improved antibiotics and other drugs has come a long way, both groups have been optimistic and have also been able to explain the structure and mechanism of the missing parts of molecular factories. “We already have promising data for additional protein complexes,” says Maximilian Schmalhofer, a PhD candidate involved in research at Munich.

This work was funded by the German Forschungs Mainshaft (DFG), with the excellence of the SPP 1617, SFB 1035, and the Integrated Protein Science Munich (CIPSM) cluster, and LOEWE’s focus on MegaSyn, Hesse. Supported X-ray structural data were measured at the Paul Scherrer Institute in Villigen (Switzerland). The Swedish national computing infrastructure provided computing time for theoretical modeling.


See journal:

Broyer, A. , other (2020) Structural snapshot of the minimal PKS system involved in octaketide biosynthesis. Natural chemistry..


What Are The Main Benefits Of Comparing Car Insurance Quotes Online

LOS ANGELES, CA / ACCESSWIRE / June 24, 2020, / Compare-autoinsurance.Org has launched a new blog post that presents the main benefits of comparing multiple car insurance quotes. For more info and free online quotes, please visit https://compare-autoinsurance.Org/the-advantages-of-comparing-prices-with-car-insurance-quotes-online/ The modern society has numerous technological advantages. One important advantage is the speed at which information is sent and received. With the help of the internet, the shopping habits of many persons have drastically changed. The car insurance industry hasn't remained untouched by these changes. On the internet, drivers can compare insurance prices and find out which sellers have the best offers. View photos The advantages of comparing online car insurance quotes are the following: Online quotes can be obtained from anywhere and at any time. Unlike physical insurance agencies, websites don't have a specific schedule and they are available at any time. Drivers that have busy working schedules, can compare quotes from anywhere and at any time, even at midnight. Multiple choices. Almost all insurance providers, no matter if they are well-known brands or just local insurers, have an online presence. Online quotes will allow policyholders the chance to discover multiple insurance companies and check their prices. Drivers are no longer required to get quotes from just a few known insurance companies. Also, local and regional insurers can provide lower insurance rates for the same services. Accurate insurance estimates. Online quotes can only be accurate if the customers provide accurate and real info about their car models and driving history. Lying about past driving incidents can make the price estimates to be lower, but when dealing with an insurance company lying to them is useless. Usually, insurance companies will do research about a potential customer before granting him coverage. Online quotes can be sorted easily. Although drivers are recommended to not choose a policy just based on its price, drivers can easily sort quotes by insurance price. Using brokerage websites will allow drivers to get quotes from multiple insurers, thus making the comparison faster and easier. For additional info, money-saving tips, and free car insurance quotes, visit https://compare-autoinsurance.Org/ Compare-autoinsurance.Org is an online provider of life, home, health, and auto insurance quotes. This website is unique because it does not simply stick to one kind of insurance provider, but brings the clients the best deals from many different online insurance carriers. In this way, clients have access to offers from multiple carriers all in one place: this website. On this site, customers have access to quotes for insurance plans from various agencies, such as local or nationwide agencies, brand names insurance companies, etc. "Online quotes can easily help drivers obtain better car insurance deals. All they have to do is to complete an online form with accurate and real info, then compare prices", said Russell Rabichev, Marketing Director of Internet Marketing Company. CONTACT: Company Name: Internet Marketing CompanyPerson for contact Name: Gurgu CPhone Number: (818) 359-3898Email: [email protected]: https://compare-autoinsurance.Org/ SOURCE: Compare-autoinsurance.Org View source version on accesswire.Com:https://www.Accesswire.Com/595055/What-Are-The-Main-Benefits-Of-Comparing-Car-Insurance-Quotes-Online View photos

Pictures Credit


to request, modification Contact us at Here or [email protected]