Health
Study: Only 1 of 32 COVID survivors tested positive had the live virus
A JAMA Internal Medicine Research letter Today, 18% of recovered COVID-19 patients were found to be positive for SARS-CoV-2 (the virus that causes COVID-19), but only a few have replication viruses in the airways. It is 3% (1 out of 32 people).
Italian researchers studied 176 recovered COVID-19 patients admitted for post-acute follow-up treatment at Fondazione Policlinico Universitario A. Gemelli IRCCS in Rome from April 21 to June 18. , Symptom improvement, and virus two negative reverse transcriptase-polymerase chain reaction (RT-PCR) test results (24-hour intervals).
Nasal / oropharyngeal (NOS) cotton swab samples from patients were analyzed for total (genome) and replication (subgenome) SARS-CoV-2 RNA using RT-PCR assays. Subgenomic RNA is not yet packaged in the fully infectious form of the virus, virions, and is transcribed only in infected cells, indicating active infection and potential for infection. Serological tests for immunoglobulin (Ig) A and IgG antibodies were also performed as part of the follow-up.
One patient shows evidence of RNA replication
Of the 176 NOS samples, 32 (18.2%) were positive for genomic SARS-CoV-2 RNA and viral load ranged to 1.6 × 10.1 Up to 1.3 x 10Four SARS-CoV2RNA copy per milliliter.
The average time from diagnosis to follow-up for SARS-CoV-2 positive patients was 48.6 days. Only one of the 32 positive samples (3.1%) had a replicative subgenome SARS-CoV-2 RNA.
All but one of the 32 SARS-CoV-2 positive patients were positive on serological tests at follow-up, and 139 of the remaining 144 patients were positive. Patients who were negative for SARS-CoV-2 antibody were not those who were positive for replication SARS-CoV-2 RNA.
“This study emphasizes that many patients who have recovered from COVID-19 may still be positive for SARS-CoV-2 RNA, but have replicated SARS-CoV-2 in the respiratory tract. Very few patients have it. Tract, “the author writes.
Currently, there are no widely available tests to determine virus replication and infectivity, and test whether recovered patients who test positive for SARS-CoV-2 can infect the virus. It emphasizes the need for further research to do so.
Problems with repeated PCR follow-up
Repeated testing of SARS-CoV-2 in recovered patients is complicated by sensitive RT-PCR testing that can detect non-viable debris of the virus, leading to unnecessary quarantine and reinfection concerns.
so editorial In the same journal, Dr. Mitchell H. Katz, MD, of New York City Health Hospital, said regular repeated PCR tests after recovery in light of PCR sensitivity and infectious uncertainty in people who continue to test positive for the virus. I am against.
“Reinfection with SARS-CoV-2 is documented (based on demonstrating different genetic differences between viruses that infect humans in episodes 1 and 2), but is rare,” Katz wrote. I have. “Until the laboratory gains the ability to test the fertility of the coronavirus, the interpretation of the epidemiological significance of positive PCR results among recovered patients remains challenging.”
reference:
August 24 CIDRAP News Article “COVID-19 has more than 23 million cases due to established evidence of reinfection“”
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