Health
covid-19: The new smartphone-based Covid-19 test gives results in less than 30 minutes, the study says
According to a study published in the journal Cell, the new diagnostic test not only produces positive or negative results, but also Viral load -The concentration of virus in a particular sample.
Researchers say that all CRISPR diagnostics to date require the viral RNA to be converted to DNA and amplified before it can be detected, increasing time and complexity.
In contrast, the new approach uses CRISPR to detect viral RNA directly, skipping all conversion and amplification steps.
“One of the reasons we’re excited about CRISPR-based diagnostics is that we can get fast and accurate results when we need them,” said Jennifer Doudna, senior researcher at the Gladstone Institutes in the United States. Donna says.
“This is especially useful in places where access to tests is restricted or when frequent and rapid testing is required. It can eliminate many of the bottlenecks seen in COVID-19,” Doudna said. Mr. said.
Doudna was awarded the 2020 Nobel Prize in Chemistry for co-discovering the underlying technology of this research, CRISPR-Cas genome editing.
In a new test, the Cas13 protein is combined with a reporter molecule that fluoresces when cleaved and then mixed with a patient sample from a nasal swab, the researchers said.
The sample will be placed on the device that connects to your smartphone. They said that if the sample contained RNA from SARS-CoV-2, Cas13 would be activated, the reporter molecule would be cleaved, and a fluorescent signal would be emitted.
According to researchers, smartphone cameras that are essentially converted to microscopes can detect fluorescence and report that swabs have been tested positive for the virus.
They say the assay can be adapted to a variety of mobile phones, providing easy access to technology.
When scientists tested the device with patient samples, they found that the turnaround times of clinically relevant viral load samples were very fast.
Studies have shown that the device accurately detected a series of positive samples within 5 minutes.
For low viral load samples, the device took up to 30 minutes to distinguish it from the negative test, the researchers said.
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