Health
New COVID-19 test with results within 5 minutes
Do I have to wait hours to get the results of the COVID-19 test? Not only is it frustrating for patients, but delays in the delivery of test results can lead to further spread of the disease, as some people do not see it. Symptoms Of COVID-19. On the other hand, quick tests and quick results give you faster insight into whether the virus needs to be quarantined to prevent it from infecting others.
Thanks to researchers at the University of Birmingham, people are now able to get COVID-19 test results within 5 minutes. They reduced the test time from 30 minutes to less than 5 minutes and designed a new test method that provided accurate results. The new COVID-19 test is documented in a preprinted paper published on MedRxiv.
Professor Tim Duffon of the University’s Department of Life Sciences states that the new test method combines the ease and speed of lateral flow testing with the inherent sensitivity of RNA testing.
It features reagents that can be used with existing point-of-care devices, meeting the need to test in high-throughput, patient-friendly environments where people may wait for results.
How is it different from the current COVID-19 inspection method?
The reverse transcription polymerase chain reaction (RT-PCR) test is the current gold standard. COVID-19 testEach sample takes more than an hour and contains two steps. The first step requires the conversion of RNA to DNA using reverse transcriptase, which usually takes 30 minutes. The second step is to use DNA polymerase enzyme to copy the DNA and amplify it to a detectable level. This requires a time-consuming heating and cooling cycle. Even a single temperature process could reduce this step to less than 20 minutes. Antibody test, It can take up to 30 minutes to determine if you are infected with a coronavirus.
To enable rapid COVID-19 testing, researchers at the University of Birmingham have developed a new method called the “reverse transcriptase-free EXPAR (RTF-EXPAR) test”. They created a new single-step approach for converting viral RNA to DNA and combined it with a known technique called exponential amplification reaction (EXPAR). This increases the DNA concentration to a detectable level at a constant temperature.
The University of Birmingham Enterprise has reportedly filed a patent for a new method and its use in diagnostic equipment. We are also trying to obtain a patent license for rapid product development.
Learn more about the new RTF-EXPAR test
According to researchers, a new test method is an enzyme that recognizes and binds to SARS-CoV-2 viral RNA (called binder DNA) and, in the case of viruses, a short section from it. BstNI) is used. RNA is present. This cycle repeats as the viral RNA binds to more binder DNA. The output of this cycle shows the results of the test.
When researchers tested the method, they obtained test results in 4.00 +/- 0.72 minutes for positive samples. The concentration of SARS-CoV-2 RNA in the positive sample was found to be 72.7 copies / µL. This is up to one-tenth the average viral load found in COVID-19 patient samples.
Although this method was developed for the COVID-19 test, researchers say it can be applied to any RNA-based infectious agent or disease biomarker, including cancer.
Release date: January 7, 2021 14:39
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