Health
The test detects SARS-CoV-2 virus even after mutation
A recent study conducted by NTU researchers in collaboration with NTU Singapore describes a diagnostic test that has been developed. SARS-CoV-2 Even after it has undergone a mutation.The results of the study were published in the journal Nature Communications..
“The virus is very smart. It can mutate, edit, or shuffle genetic material, which may prevent diagnostic tests from detecting the virus,” said research leader Tammenhow. High-sensitivity test that can catch the virus even if the gene sequence changes In addition, frequent tests are indispensable to prevent the transmission of the virus in the population, so develop the test quickly and affordably and use it as a resource Allowed to be deployed-insufficient settings. “
The researchers behind the study used a reaction mixture containing enAsCas12a, a variant of the enzyme Cas12a, which acts like a pair of “molecular scissors” to extract a segment of the SARS-CoV-2 virus from the rest of the viral genome. I programmed it to cut. This is done by two different molecules known as guide RNAs designed to recognize specific sites on the viral genome.
“Combining two or more guide RNAs with the enzyme enAsCas12a allows other guide RNAs to’relieve’this mismatch, even if one of the guide RNAs cannot guide to the correct segment of the virus due to mutations,” How said. Says. ..
When a virus or variant is detected, the Cas12 enzyme is overactivated and begins to cut out detectable genetic material in the sample, including fluorochrome-tagged molecules that are added to the reaction mixture. When this molecule is cleaved, it glows and is detected by a microplate reader.
A test named VaNGuard (Variant Nucleotide Guard) is so far capable of recognizing up to two mutations within a target site on the SARS-CoV-2 genome.
“If the virus is present, the molecule will glow. If not, it means that the virus is absent, causing overactivation of the molecule scissors.”
The team is not only planning future research to further improve the diagnostic kit, but has also obtained approval from relevant authorities to commercialize the test.
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