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Why Some Patients Are COVID Positive After Recovery | Health News
New York: Some viral RNAs may be reverse transcribed and inserted into the human genome, and new studies have been found. This may explain why some people who have already recovered from COVID-19 may be mysteriously positive on the PCR test after weeks or months.
Researchers at the Whitehead Institute of Biomedical Sciences and the Massachusetts Institute of Technology (MIT) in the United States have shown that the gene sequence of the RNA virus SARS-CoV-2 is integrated into the host cell’s genome through a process called reverse transcription. .. You can then “read” these sections of the genome into RNA. This can be detected by PCR tests. The results are published online in the National Academy of Sciences journal Proceedings6.
SARS-CoV-2 is not the only virus that integrates into the human genome. About 8% of our DNA is made up of the remains of ancient viruses. Some viruses, called retroviruses, rely on integration into human DNA to replicate themselves.
“SARS-CoV-2 is not a retrovirus, which means it does not require reverse transcription for replication,” said Liguo Zhang, a postdoctoral fellow at Whitehead Institute.
“However, nonretroviral RNA viral sequences have been detected in the genomes of many vertebrate species, including humans,” Zhang added.
With this in mind, the team analyzed public datasets of RNA transcripts from different types of samples, including COVID-19 patient samples, and could this viral integration occur with the new coronavirus? I tested it.
They were able to calculate the percentage of genes transcribed in the cells of these patients, including viral sequences that may be derived from the integrated viral copy.
The proportions vary from sample to sample, but it seems that a relatively large portion of some viral transcripts were transcribed from the viral genetic material integrated into the genome.
In the case of SARS-CoV-2, the frequency of human integration is still unknown. “The percentage of cells that integrate can be very small,” said Rudolf Jaenisch, a professor of biology at MIT.
“But, rarely, more than 140 million people are already infected, right?” Jaenisch said.
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