Health
NIH scientists are developing Covid-19 tests that are faster than RT-PCR
Scientists at the National Institutes of Health (NIH) have developed a new sample preparation method for detecting SARS-CoV-2. This method may bypass the extraction of viral gene RNA material, reducing test time and cost.
This method is the result of collaborative research between researchers at the National Eye Institute (NEI), NIH Clinical Center (CC), and the National Dental Cranioface Research Institute (NIDCR).
Standard tests use a technique called RT-qPCR to amplify viral RNA to detectable levels. However, first you need to extract the RNA from the sample. Manufacturers of RNA extraction kits Pandemic..
Researchers used a drug called “Chelex 100 Resin” manufactured by lab supplier Bio-Rad to store SARS-CoV-2 RNA in samples for detection by RT-qPCR.
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“We used nasopharyngeal and saliva samples with different virion concentrations to assess whether they could be used directly for RNA detection. The answer was yes, which was very sensitive. Also, this preparation inactivated the virus. This has made it safer for lab personnel to process positive samples, “NIH quoted Bin Guan, lead author of the National Eye Institute. This paper was published in iScience.
The team tested a variety of chemicals using synthetic and human samples to enable direct detection of the virus by RT-qPCR, while minimizing degradation and preserving RNA in the sample. I found it by identifying it.
To validate the test, they collected patient samples and stored them in either viral transport media or in newly developed chelated resin buffers at the NIH Symptomatic Testing Facility.
Samples of virus transport medium COVID-19 NIH Clinical Center test team using traditional RNA extraction and RT-qPCR tests. Samples in chelated resin buffer were heated and viral RNA was tested by RT-qPCR. The new preparation significantly increased the RNA yield available for testing compared to standard methods. — —Source: NIH (USA)
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