Study reports C. vulturna outbreak in Chinese hospital

In a recent study published in emerging infectious diseasethe researchers Candida varturna Number of infected people living in China from 2019 to 2022.

Background

C. Balturnaphylogenetically similar fungal organisms Candida auris and Candida hemroni, was first obtained from flowers in 2016 and has since been isolated sporadically in several countries. Fungal pathogens have been isolated from living tissue such as serum and non-living agents such as peripherally inserted central catheters (PICCs).

Candida auris, Candida hemroniand Candida varturna derived from mechnikova clade. Resistance to antifungal agents such as azoles is commonly reported among species in this clade. In mainland China, white ears Infections are less frequent. However, other Candida species, Candida hemroni It’s becoming more and more popular these days.

About research

In this study, researchers evaluated anonymized data obtained from hospitalized individuals in Shanxi, China. Candida varturna Infection.

The team selected 19 infected individuals (17 males and 2 females). Candida varturna From January 1, 2019 to October 26, 2022. Cell culture experiments were performed using a chromogenic device. culture Media and liquid media for identifying growth of Candida species. This was verified by sequencing the internal transcribed spacer (ITS) site within the ribosome.

The fungal isolates were phylogenetically analyzed based on ITS sequences and genotyping of multilocus sequences of eight conserved genes. In addition, an environmental screening test was performed to detect Candida on hospital surfaces, including floors, walls, bed sheets, bedside tables, bed frames, chairs, and blood pressure cuffs.

In addition, phenotypic analysis and antifungal susceptibility testing were performed using one representative strain from each patient to determine minimum inhibitor concentration (MIC) values ​​expressed in mg per liter and The antifungal agent of choice was determined based on the cut-off to be taken. Candida albicans.

result

Of the study participants, 11 were admitted to the intensive care unit (ICU), 4 to the neuroscience department, and 4 to other departments. Participants’ ages ranged from 13.0 to 83.0 years.Team separated 16 Candida varturna Bloodstream strains (from blood samples obtained by venipuncture) and seven Candida varturna Distortion from peripherally inserted central catheter tips in all patients.

The stock was originally identified as belonging to: Candida hemroni Complex based on chromogenic growth properties culture Moderate and verified by ITS sequencing. The majority of cases were discovered in 2019. Candida varturna Two patients were confirmed to be infected between 1 January 2020 and 1 January 2022, the peak period for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases in the region. it was done. Increased sanitary precautions taken during this period may have reduced the number of cases. C. Balturna Infection in the nosocomial environment.

All detected fungal strains (CVDH1–19) clustered in the same clade, showing phylogenetic similarity. As PICCs were used to deliver drugs to all study participants, Candida varturna The use of PICC lines could be considered a risk-increasing factor, as the use of PICC lines was detected at the tip of peripherally inserted central catheters in 7 patients. Candida varturna Infection.

team could not be detected Candida varturna on hospital surfaces. Candida varturna Resistant to azoles and amphotericin B (MIC 4.0), but not flucytosine (minimum inhibitory concentration 0.1) and caspofungin (MIC ≤ 0.10), anidulafungin (MIC ≤ 0.10), and micafungin (MIC ≤ 0.50) showed sensitivity to echinocandins such as

In liquid medium, Ci love valturna Cells formed large aggregates with improved adhesion and enhanced ability to form biofilms.Properties were similar to SJ01 Candida ear This strain showed enhanced biofilm formation in the body. in vitro Even if live setting.

Conclusion

In conclusion, based on research results, there are novel multidrug-resistant fungi such as: Candida varturnaof, mechnikova Clades with enhanced adhesion and enhanced biofilm-forming capacity could threaten public health and increase the burden on healthcare facilities. Widespread antifungal drug prescribing by clinicians, use of fungicides by farmers, and declining susceptibility of new species to antifungal drugs are contributing to the increasing emergence of multidrug-resistant pathogenic fungal organisms. There is a possibility.

Findings indicated the need for rigorous disinfection.did it Prevent candida varturna contagion; infection.many Candida varturna Strains were isolated from the blood of individuals, indicating that strains that caused outbreaks were phylogenetically related and likely had a common ancestral origin. The isolated fungal strains were less sensitive to amphotericin B and azoles. Candida hemroni Race. Use of PICC circuits may increase the risk of: Candida varturna It includes trauma, cancer, hypertension, pulmonary and blood infections, as well as infections.