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Researchers say new portable technology can detect antiviral antibodies in 20 minutes
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According to Japanese researchers, the new technology can detect antiviral antibodies in just 20 minutes.
If a suitable reagent was developed, they would use that technique for SARS-CoV-2, COVID-19 (New Coronavirus Infection) (# If there is no character limit, add parentheses when first appearing.
Ah reagent A substance used to detect or measure other substances.
A portable analyzer operating with a small microfluidic device detected anti-avian influenza virus antibody in serum, statement Released by Hokkaido University.
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“Once we have developed the right reagents, we can also use our analyzers to perform other biotests,” said Professor Tokushi of Hokkaido University in a statement. “By replicating the spike protein fragments expressed by the new coronavirus and using them as reagents, the analyzer should be able to detect anti-coronavirus antibodies.”
A small microfluidic device used to detect antiviral antibodies on an analyzer.
(Kenichi Nishiyama et al., Sensor and Actuator B: Chemical, April 21, 2020)
Research papersSensors and Actuators B: Chemistry. “
According to data from Johns Hopkins University, as of Wednesday morning, there were 16,623 coronavirus cases diagnosed in Japan with 846 deaths.
Researchers at Hokkaido University have pointed out that the polymerase chain reaction (PCR) method is commonly used to detect viral genomes. But they say this is a complicated procedure that can take quite some time. New method uses traditional fluorescence polarization immunoassay (FPIA) Harness Fluorescent dye and light to detect antibodies.
But since it weighs over 12 pounds, researchers say they are using another measurement mechanism to make the analyzer device small and portable.
The portable fluorescence polarization analyzer combines a microfluidic device, an optical system, a liquid crystal, and an image sensor.
(Kenichi Nishiyama et al., Sensor and Actuator B: Chemical, April 21, 2020)
“By using a combination of liquid crystal molecules, image sensors, and microfluidic devices, it is possible to test multiple samples simultaneously, reducing the amount of each sample needed,” they said. Mentioned in. “Liquid crystal molecules can control the polarization direction of fluorescent lamps, but microfluidic devices have a large number of microchannels as measuring vessels.”
Scientists have also developed a reagent to detect anti-H5 avian influenza virus antibodies. Serum from birds was mixed with reagents and left for 15 minutes, they said in a statement. The mixture was then injected into the developed microfluidic device and measured with a portable fluorescence polarization analyzer.
“The molecular motion of the reagent bound to the antibody is reduced in the liquid, producing a different degree of polarization than the reagent not bound to the antibody,” they said.
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Images taken by the device-Samples have different degrees of polarization of light depending on the presence or amount of anti-H5 avian influenza virus antibodies.
(Kenichi Nishiyama et al., Sensor and Actuator B: Chemical, April 21, 2020)
According to data from Johns Hopkins University, as of Wednesday morning, more than 5.66 million cases of coronavirus were diagnosed worldwide, of which at least 1.68 million were in the United States. The disease causes more than 350,000 deaths worldwide, with at least 98,929 deaths in the United States.
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