Health
Nanomedicines can be improved with appropriate and more predictive in vitro assays
Messenger RNA (mRNA) vaccines are a prime example of a promising field of nanomedicine.However, advances in the design and application of nanoparticles as efficient delivery media for biopharmaceutics, including: Nucleic acid Or protein drug substances are, unfortunately, significantly slower.
One of the recent obstacles to drug delivery research is the observation of a weak correlation between in vitro and in vivo performance. This problem was not apparent in the early stages when cell delivery of drug nanocarriers was tested primarily in standard cell cultures. Advanced pharmacological in vivo studies emerging in mouse or human patients are revealing the low reliability and validity of cell culture tests for therapeutic applications.
Intravenous administration of nanoparticles presents some obstacles that differ from the in vitro situation, such as contact with blood components. Nanoparticles are usually covered with a multi-layered biomolecule (protein corona). This changes the physicochemical properties, pharmacokinetics, and toxicity profile of nanoparticles.
In AIP Publishing’s Biophysics Reviews, German researchers provide state-of-the-art characterization of the protein corona formed around nanoparticles and their impact on the physicochemical and biological properties of these nanoparticles. ..
When predicting in vivo performance from in vitro data, it is recommended to combine several analytical and biological characterization methods to gain more detailed insight into the in vivo properties and behavior of nanoparticles. “
Simone Berger, co-author, Ludwig Maximilian University, Munich
Selection of biofluids (serum, plasma, or whole blood, and animals of origin) and establishment of standardized protocols are more consistent, robust, and comprehensive for deriving structural activity correlations and in vitro /. Very important for preclinical trials. In vivo correlation.
“The knowledge gained about protein corona formation can be used to optimize carriers for nanomedical applications,” says Berger.
Researchers have pointed out that information such as in vivo biodistribution and off-target effects cannot be obtained from in vitro experiments. However, new high-throughput screening methods such as barcode systems can make in vivo research more effective, economical, and ethical.
Although some uncertainty remains about the translatability from small animals to large animals and humans, bioinformatics can help identify the optimal animal model for a particular disease.
“Animal model alternatives, such as microfluidic’human organ-on-chip’technology and computational predictions, could be promising strategies to replace animal testing in the future,” Berger said.
Nanomedicines “show great potential to revolutionize the therapeutic environment in a wide range of applications, including cancer vaccines / immunotherapy and the treatment of hereditary diseases,” Berger said. “Appropriate and more predictive in vitro assays make preclinical pipelines more efficient, rapid, and economical, and, importantly, can replace or at least reduce animal testing.”
Source:
Journal reference:
Burger, S. , et al. (2022) Nanoparticle performance for biomedical applications: in vitro / in vivo discrepancies. Biophysics review. doi.org/10.1063/5.0073494..
|
Sources 2/ https://www.news-medical.net/news/20220201/Nanomedicine-can-be-improved-with-proper-and-more-predictive-in-vitro-assays.aspx The mention sources can contact us to remove/changing this article |
What Are The Main Benefits Of Comparing Car Insurance Quotes Online
LOS ANGELES, CA / ACCESSWIRE / June 24, 2020, / Compare-autoinsurance.Org has launched a new blog post that presents the main benefits of comparing multiple car insurance quotes. For more info and free online quotes, please visit https://compare-autoinsurance.Org/the-advantages-of-comparing-prices-with-car-insurance-quotes-online/ The modern society has numerous technological advantages. One important advantage is the speed at which information is sent and received. With the help of the internet, the shopping habits of many persons have drastically changed. The car insurance industry hasn't remained untouched by these changes. On the internet, drivers can compare insurance prices and find out which sellers have the best offers. View photos The advantages of comparing online car insurance quotes are the following: Online quotes can be obtained from anywhere and at any time. Unlike physical insurance agencies, websites don't have a specific schedule and they are available at any time. Drivers that have busy working schedules, can compare quotes from anywhere and at any time, even at midnight. Multiple choices. Almost all insurance providers, no matter if they are well-known brands or just local insurers, have an online presence. Online quotes will allow policyholders the chance to discover multiple insurance companies and check their prices. Drivers are no longer required to get quotes from just a few known insurance companies. Also, local and regional insurers can provide lower insurance rates for the same services. Accurate insurance estimates. Online quotes can only be accurate if the customers provide accurate and real info about their car models and driving history. Lying about past driving incidents can make the price estimates to be lower, but when dealing with an insurance company lying to them is useless. Usually, insurance companies will do research about a potential customer before granting him coverage. Online quotes can be sorted easily. Although drivers are recommended to not choose a policy just based on its price, drivers can easily sort quotes by insurance price. Using brokerage websites will allow drivers to get quotes from multiple insurers, thus making the comparison faster and easier. For additional info, money-saving tips, and free car insurance quotes, visit https://compare-autoinsurance.Org/ Compare-autoinsurance.Org is an online provider of life, home, health, and auto insurance quotes. This website is unique because it does not simply stick to one kind of insurance provider, but brings the clients the best deals from many different online insurance carriers. In this way, clients have access to offers from multiple carriers all in one place: this website. On this site, customers have access to quotes for insurance plans from various agencies, such as local or nationwide agencies, brand names insurance companies, etc. "Online quotes can easily help drivers obtain better car insurance deals. All they have to do is to complete an online form with accurate and real info, then compare prices", said Russell Rabichev, Marketing Director of Internet Marketing Company. CONTACT: Company Name: Internet Marketing CompanyPerson for contact Name: Gurgu CPhone Number: (818) 359-3898Email: [email protected]: https://compare-autoinsurance.Org/ SOURCE: Compare-autoinsurance.Org View source version on accesswire.Com:https://www.Accesswire.Com/595055/What-Are-The-Main-Benefits-Of-Comparing-Car-Insurance-Quotes-Online View photos
to request, modification Contact us at Here or [email protected]
 |
 |
 |
