Study Results May Improve Diagnosis of Vitamin D Deficiency

The results of new research may lead to a more accurate diagnosis of vitamin D deficiency. Researchers from the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC-IOF) and the International Osteoporosis Foundation Present Findings to Determine Analytical Performance Specifications for the 24,25-Dihydroxyvitamin D (24,25(OH)2D) Test bottom.¹

Valery Honcharuk – Stock.adobe.com

According to the researchers, the researchers investigated metabolites in the vitamin D degradation pathway, which has become increasingly important in recent years. Simultaneous quantitation of 25-hydroxyvitamin D (25(OH)D) and 24,25(OH)2D has been proposed as a new approach to define vitamin D deficiency, but it is not available for 24,25(OH). No data available. ) 2D biological variation so far.¹

“This study advances our understanding of the degradation pathways of vitamin D in healthy humans and, importantly, defines for the first time analytical performance specifications for 24,25(OH)2D tests. Given the upsurge, some laboratories and manufacturers may aim to develop specific methods for its measurement, and thus these findings represent a necessary prerequisite for its validation.” The Chair of the IFCC-IOF Bone Metabolism Committee said in a statement:¹

In this study, researchers evaluated biological mutations in 24,25(OH)2D using European Biological Variation Study cohort samples. They aimed to determine whether analytical performance specifications for 24,25(OH)2D could be generated.¹

Six European laboratories, a total of 91 healthy people participated in the study. Concentrations of 24,25(OH)2D and 25(OH)D in EDTA plasma were examined weekly for up to 10 weeks using validated liquid chromatography and mass spectrometry methods.¹

The researchers calculated the vitamin D metabolite ratio by dividing 24,25(OH)2D by 25(OH)D and multiplying by 100 at each time point.¹

This finding was demonstrated by linear regression of the average 24,25(OH)2D concentration in each blood sample to indicate individuals not in steady state. Over time, variation in 24,25(OH)2D was significantly positively correlated with the slope of her 25(OH)D concentration over time and with the individual’s 25(OH)D concentration at baseline. There was a relationship.¹

Furthermore, it was found that there was a negative correlation with BMI.¹

The variation in individual 24,25(OH)2D concentrations over 10 weeks was 34.6%. A method to detect significant changes associated with spontaneous generation of 24,25(OH)D over this period should have a relative measurement uncertainty of less than 14.9%. For p<0.01, the measurement uncertainty should be less than 10.5%.¹

“This study demonstrates the tremendous value of international collaboration, bringing together key expertise of IFCC and IOF, and establishes key technical parameters that facilitate clinical validation. [24,25(OH)2D] Nicholas Harvey, Director of the University of Southampton MRC Center for Life Course Epidemiology and Chair of the IOF Scientific Advisory Board, said in a statement.¹

Data from the National Health and Nutrition Examination Survey show that between 2001 and 2006, about two-thirds of the population had adequate vitamin D levels, one-quarter were at risk of vitamin D deficiency,8 % were at risk of vitamin D deficiency.²

In addition, the risk of vitamin D deficiency increased between 1988 and 1994 and between 2001 and 2002.²

reference

1. A new study defines analytical performance specifications for the 24,25-dihydroxyvitamin D test. news release. Yulek Alert. May 23, 2023. Accessed June 9, 2023. https://www.eurekalert.org/news-releases/990133

2. Vitamin D Status: United States, 2001-2006. Centers for Disease Control and Prevention. March 2011. Updated 11/6/2015. Accessed June 8, 2023. https://www.cdc.gov/nchs/products/databriefs/db59.htm